Maciej Pilarek, Ph.D., Eng.

Warsaw University of Technology
Faculty of Chemical and Process Engineering
ul. Warynskiego 1
00-645 Warsaw
Poland

e-mail: pilarek@ichip.pw.edu.pl
tel.: ++48-22-2346272
Skype: maciekp
homepage: www.ichip.pw.edu.pl/pilarek

Employment History:

from October 2008: Warsaw University of Technology, Faculty of Chemical and Process Engineering

2004-2008: Warsaw University of Technology, Centre for Biotechnology

Education:

2009.10-2010.01: Visiting scientist
Technische Universitat Berlin, Institute for Biotechnology, Department of Bioprocess Technology

1999-2003: Ph.D. Studies in Biochemical Engineering
Warsaw Univeristy of Technology, Faculty of Chemical and Process Engineering
Ph.D. thesis (October 2003): "Studies on kinetics of enzymatic alcoholysis of triacylglycerols"

1994-1999: Graduated M.Sc., Eng. Studies in Industrial Biotechnology
Warsaw University of Technology, Faculty of Chemical and Process Engineering
M.Sc. thesis (June 1999): "Biochemical properties of chitin deacetylase from Absidia orchidis"

Research activities

- perfluorochemicals (fluorocarbons, PFC) as oxygen carriers in cell cultures

- bioreactors for plant and animal cell cultures
- enzymatic alcoholysis of lipids

Patents

Pilarek M., Neubauer P.: "Method for increasing the expression of a recombinant protein and plasmid yield in an eukaryotic and prokaryotic high cell density culture"; European Patent Office EP10168159. Filing date 01.07.2010.

Publications:

Pilarek M., Brand E., Hillig F., Neubauer P.: "Microscale high cell density Escherichia coli cultures supported with a liquid oxygen carrier for enhanced plasmid production", Acta Biochimica Polonica 58 Supplement 4 (2011), 5.

Pilarek M., Kaczmarczyk M., Olak S., Grabowska I., Szewczyk K.W.: "Cultures of 3-D aggregated mammalian cells in a two-phase liquid/liquid system containing perfluorinated carrier of respiratory gases", Acta Biochimica Polonica 58 Supplement 4 (2011), 5.

Pilarek M., Glazyrina J., Neubauer P.: "Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures", Microbial Cell Factories 10 (2011), 50.

Pilarek M., Neubauer P., Marx U.: "Biological cardio-micro-pumps for microbioreactors and analytical micro-systems", Sensors and Actuators B: Chemical 156 (2011), 517-526.

Pilarek M., Chaber E., Szewczyk K.W.: "Perfluorinated systems for culture of animal cells", Inzynieria i Aparatura Chemiczna 48 (2009), 83-84 (in Polish).

Pilarek M., Dębowska J., Szewczyk K.W.: "Culture of Nicotiana tabacum BY-2 cells in a membrane bioreactor with perfluorinated oxygen carrier", Inzynieria i Aparatura Chemiczna 48 (2009), 85-86 (in Polish).

Pilarek M., Szewczyk K.W., Chaber E.: "Animal cell cultures on liquid/liquid interfacial area", Acta Biochimica Polonica 4-Supplement (2008), 54.

Pilarek M., Szewczyk K.W.: "Effects of perfluorinated oxygen carrier application in yeast, fungi and plant cell suspension cultures", Biochemical Engineering Journal 41 (2008), 38-42.

Pilarek M., Szewczyk K.W.: "Application of a liquid oxygen carrier in plant cell suspension cultures", Journal of Biotechnology 131 (2007), S143.

Pilarek M., Szewczyk K.W.: "Kinetic model of 1,3-specyfic triacylglycerols alcoholysis catalyzed by lipases", Journal of Biotechnology 127 (2007), 736-744.

Pilarek M., Szewczyk K.W., Stepniewski J., Anderszewska A.: "The use of a perfluorinated oxygen vector in cultures of microorganisms", Przemysł Chemiczny / Chemical Review 85 (2006), 1131-1133 (in Polish).

Pilarek M., Szewczyk K.W., Stepniewski J.: "Perfluorodecalin as an oxygen vector in Saccharomyces cerevisiae cultures", Inzynieria i Aparatura Chemiczna 44 (2005), 76-78 (in Polish).

Pilarek M., Szewczyk K.W.: "Perfluorochemicals as liquid vectors of respiratory gases used in medicine and biotechnology" Biotechnologia 69 (2005), 125-150 (in Polish).

Pilarek M., Szewczyk K.W.:, "Kinetic model of enzymatic tricaprylin isopropanolysis" Inzynieria Chemiczna i Procesowa / Chemical and Process Engineering 25 (2004), 1495-1501 (in Polish).

Szewczyk K.W., Pilarek M., Tymosiewicz R., Pec K.: "The effect of fatty acid chain length on kinetics of enzymatic alcoholysis" Przemysł Chemiczny / Chemical Review 82 (2003), 1100-1103 (in Polish).

Pilarek M., Szewczyk K.W.: "Enzymatic isopropanolysis of triacylglycerols" Inzynieria i Aparatura Chemiczna 41 (2002), 116-117 (in Polish).

Pilarek M., Szewczyk K.W., Wrona M.: "Trends and perspectives of lipases applications" Biotechnologia 57 (2002), 146-164 (in Polish).

Szewczyk K.W., Pilarek M., Wrona M.: "Enzymatic propanolysis of triacetin" Inzynieria Chemiczna i Procesowa / Chemical and Process Engineering 22 (2001), 1351-1356 (in Polish).

Jaworska M. M., Pilarek M., Konieczna E.: "Chitin deacetylase from Absidia orchidis", Progress on Chemistry and Application of Chitin and Its Derivates 7 (2001), 79-86.

Congress and conference presentations:

"Microscale high cell density Escherichia coli cultures supported with a liquid oxygen carrier for enhanced plasmid production" - poster at 4th Congress of Polish Biotechnology and 4th European Congress of Life Sciences EUROBIOTECH 2011, Krakow (PL) 2011.

"Cultures of 3-D aggregated mammalian cells in a two-phase liquid/liquid system containing perfluorinated carrier of respiratory gases" - poster at 4th Congress of Polish Biotechnology and 4th European Congress of Life Sciences EUROBIOTECH 2011, Krakow (PL) 2011.

"Enhanced growth and recombinant protein production of Escherichia coli by perfluorochemical applied in miniaturazed fed-batch cultures"- poster at BioProScale Symposium, Berlin (DE) 2009.

"Perfluorinated systems for culture of animal cells"- presentation at 10 Bioreactor Engineering Advances Conference, Lodz (PL) 2009.

"Culture of Nicotiana tabacum BY-2 cells in a membrane bioreactor with perfluorinated oxygen carrier" - poster at 10 Bioreactor Engineering Advances Conference, Lodz (PL) 2009.

"Animal cell cultures on liquid/liquid interfacial area" - poster at Central European Congress of Life Sciences EUROBIOTECH 2008, Krakow (PL) 2008.

"Application of a liquid oxygen carrier in plant cell suspension cultures" - poster at 13^th European Congress on Biotechnology, Barcelona (SP) 2007.

"Suspension cultures of Nicotiana tabacum BY-2 cells with perfluorinated oxygen carrier" - poster at 3^th National Congress on Biotechnology, Poznan (PL) 2007.

"The use of a perfluorinated oxygen vector in cultures of microorganisms" - presentation at 5 National Congress on Chemical Technology, Poznan (PL) 2006.

"Perfluorodecalin as an oxygen vector in Saccharomyces cerevisiae cultures" - presentation at 9 Bioreactor Engineering Advances Conference, Lodz (PL) 2005.

"Callus induction in variety of Nicotiana tabacum L." - poster at 9 Bioreactor Engineering Advances Conference, Lodz (PL) 2005.

"Kinetic model of enzymatic tricaprylin isopropanolysis" - poster at 18^th Nationwide Scientific Conference on Chemical Engineering, Szczyrk (PL) 2004.

"The effect of fatty acid chain length on kinetics of enzymatic alcoholysis" - presentation at 4 National Congress on Chemical Technology, Lodz (PL) 2003.

"Mathematical modelling of enzymatic alcoholysis of triacylglycerols" - presentation at 2^nd National Congress on Biotechnology, Lodz (PL) 2003.

"Enzymatic isopropanolysis of triacylglycerols" - presentation at 8 Bioreactor Engineering Advances Conference, Lodz (PL) 2002.

"1,3-specyfic alcoholysis of triacylglycerols" - poster at 4^th European Symposium on Biochemial Engineering Science (ESBES-4), Delft (NL) 2002.

"Enzymatic propanolysis of triacetin" - poster at 17^th Nationwide Scientific Conference on Chemical Engineering, Polanica Zdroj (PL) 2001.

"Enzymatic alcoholysis of triacetin" - poster at 10^th European Congress on Biotechnology, Madrid (SP) 2001.

Teaching activities:

Plant and Animal Cell and Tissue Cultures - obligatory lecture for Biotechnology students (6^th semester).

Biochemistry - laboratory exercises for Biotechnology students (4^th semester).

Teaching publications:

Walory J., Pilarek M., Kalinowska M., Jaworowska-Deptuch H.: "Biochemistry. Laboratory exercises", Warsaw University of Technology Publishing Department, ISBN 83-7207-393-7, Warsaw 2003 (in Polish).

"Laboratory of Bioprocesses" edited by Szewczyk K.W., Warsaw University of Technology Publishing Department, ISBN 83-7207-371-6, Warsaw 2002 (in Polish).

Other teaching and popular-science experience:

EFB RBO Warsaw Poland - Manager of European Federation of Biotechnology Regional Branch Office for Poland, Ukraine and Belarus.

KNB "Herbion" - tutor of Scientific Biotechnology Group "Herbion" at Warsaw University of Technology.

Scientific Festival and Science Picnic - lectures organizer during most known popular-science events in Poland.

SNSBio (Science Seminarium for Biotechnology Students) - organization of annual meeting for biotechnology students of various Warsaw Universities.

ONS Bio PW (Scientific Camps for Biotechnology Students) - organization and leadership of annual scientific camps for biotechnology students from Warsaw University of Technology.

;-) Leisure activities:

Planning and organization of treking and hiking trips to National Parks in Poland and tours round Europe.

Photography of nature.

GPS navigation.